關於一貼靈貼劑抗炎鎮痛作用的實驗研究

［摘要］ 目的：觀察一貼靈貼劑的抗炎鎮痛療效。方法：以阿司匹林爲對照藥物，將一貼靈貼劑用於治療二甲苯所致的小鼠耳腫脹和角叉採膠所致的大鼠足趾腫脹，以及熱和醋酸所致的小鼠疼痛，測量腫脹度、痛閾值及扭體次數。結果：一貼靈貼劑高、低劑量組及阿司匹林組耳腫脹抑制率分別爲67.92%、52.52%、58.28%；足趾腫脹抑制率比較，大小順序爲高劑量組、阿司匹林組、低劑量組；隨着劑量增加，一貼靈貼劑各劑量組小鼠痛閾值增大，扭體次數減少；一貼靈貼劑高、中劑量組小鼠的痛閾值與阿司匹林組接近。結論：一貼靈貼劑具較強的抗炎鎮痛作用。
　　［關鍵詞］ 一貼靈貼劑; 抗炎; 鎮痛
　　Experimental study on antiinflammatory and analgesic effects of Yitieling Paste
　　ABSTRACT Objective: To observe the antiinflammatory and analgesic effects of Yitieling Paste (YTLP). Methods: YTLP and aspirin (as a control drug) were introduced to treat the edematous ears of mice induced by xylene and swollen toes of mice induced by carrageenin, and to relieve the pain in mice induced by heat and acetic acid. The swelling degree, pain threshold and body distortions were measured. Results: The repression rates of the ear edema in groups of YTLP of high and low dosages and the aspirin group were 67.92%, 52.52% and 58.28%, respectively. The repression rate of the toe swelling in YTLP high dosage group was higher than that of the aspirin group, which was higher than that of the YTLP low dosage group. The results of analgesic effect of the three YTLPtreated groups showed that with the increase of dosage, the pain thresholds were higher and higher, and the body distortions were lower and lower. The pain thresholds of high and medium dosage YTLP groups were near to the aspirin group. Conclusion: YTLP possesses a strong antiinflammatory and analgesic effect.
　　KEY WORDS Yitieling Paster; antiinflammation; analgesia
　　一貼靈貼劑爲華中科技大學同濟醫學院研製的`中藥外用製劑，由細辛、當歸、川芎、莪術、桂枝等中藥組成，臨牀主要用於風溼性關節炎、骨關節炎、關節滑膜炎、類風溼性關節炎、肩周炎、風溼性肌痛、頸椎病、腰椎肥大及各種急性軟組織挫傷等，具有良好的抗炎鎮痛作用。我們對其進行了抗炎鎮痛實驗研究，茲報道如下。
　　1 材料與方法
　　1.1 動物 昆明種小鼠，雌雄按試驗要求而定，4周齡，體質量18～22 g；SD大鼠，雌雄各半，6周齡，體質量（191.0±10.3）g。均由華中科技大學同濟醫學院實驗動物中心提供。
　　1.2 藥物與試劑 細辛、當歸、川芎、莪術、桂枝等由武漢國藥集團提供；一貼靈貼劑由中藥細粉及提取物加入適量冰片、月桂氮卓酮（以下簡稱氮酮，購自湖北省恆康醫藥有限公司）和凡士林製成。1%角叉採膠由0.1 g角叉採膠加入10 ml生理鹽水配成，於4 ℃冰箱中靜置12 h，備用。放免藥盒，北京北免東雅生物技術研究所生產（批號：20030525）；阿司匹林腸溶片，江西贛江製藥有限責任公司生產（批號：20020403）；硫化鈉，天津市東麗區東大化工廠生產（批號：200208022）；脫毛劑由硫化鈉12 g、洗衣粉1 g、澱粉7 g調成糊狀而得。其他試劑爲藥用或分析純。
　　1.3 實驗方法
　　1.3.1 小鼠耳廓腫脹試驗［1］ 取小鼠40只，雌雄各半，腹部脫毛，隨機分爲4組。模型組：生理鹽水灌胃；阿司匹林組：阿司匹林灌胃，劑量爲0.2 g/kg；貼劑Ⅰ組：給藥劑量2.4 g/kg，貼於脫毛處皮膚上；貼劑Ⅱ組：給藥劑量1.2 g/kg，貼於脫毛處皮膚上。除對照組灌胃外，其餘各組動物每天貼敷2 h，連續用藥2 d。於第3天貼敷或灌胃1 h，各組動物右耳兩面塗二甲苯0.03 ml致炎。30 min後將小鼠脫臼致死，用直徑7 mm打孔器分別在左右耳對稱處取下圓形耳片，稱重，以左右耳的重量差作爲腫脹度，並計算腫脹抑制率。抑制率=(模型組腫脹度均值－用藥組腫脹度均值)/模型組腫脹度均值×100%。